heterozygous b6 sjl slc6a3 tm1 1 cre bkmn j mice  (Jackson Laboratory)

Journal: bioRxiv

Article Title: Genetic Identification of Dopamine Neurons Required for Circadian Food Anticipatory Activity in Mice

doi: 10.64898/2026.03.27.714759

Figure Lengend Snippet: A-B) Confocal imaging of immunofluorescence antibody staining of TH (magenta) and DAT (green) in WT (top) and Slc6a3 Cre ;Th Flox cKO (bottom) mice. Overlap (far right) shows co-expression (white) of these markers. C) Cell count of TH+ cells in WT (black) and Slc6a3 Cre ;Th Flox cKO (brown). D) Normalized body weight of WT and Slc6a3 Cre ;Th Flox cKO mice over the course of the study; weight was normalized by dividing the weekly weight by the Day 0 weight, which was taken prior to beginning CR. E) Raw food intake of WT and Slc6a3 Cre ;Th Flox cKO mice while on AL. F) Normalized food intake of WT and Slc6a3 Cre ;Th Flox cKO mice while on AL; data is normalized by dividing total AL raw food intake by initial body weight. G-I) Normalized high activity on days -7 (G), 21 (H), and 28 (I); data is normalized by dividing the seconds of high activity in the premeal window by the total daily high activity. J) Total daily high activity in seconds throughout the course of the study. K) High activity in seconds during the 3-hour premeal window throughout the course of the study. L) Normalized high activity during the 3-hour premeal window; data is normalized by dividing the seconds of high activity in the premeal window by the total daily high activity. Data are mean +/- SEM. Statistical significance of behavior was determined using a Mixed Effect’s Analysis with Sidak’s Multiple Comparisons Test. Statistical significance of cell counts, feeding and weight were determined using an Unpaired Two-tailed t-test with Welch’s Correction. ∗p < 0.05, ∗∗ p < 0.01, *** p < 0.001.

Article Snippet: Slc6a3 Cre -driver mice were obtained from the Jackson Laboratory (Bar Harbor, ME; stock #020080); this line contains a gene insertion of Cre recombinase upstream of Slc6a3 .

Techniques: Imaging, Immunofluorescence, Staining, Expressing, Cell Characterization, Activity Assay, Two Tailed Test

Journal: bioRxiv

Article Title: Genetic Identification of Dopamine Neurons Required for Circadian Food Anticipatory Activity in Mice

doi: 10.64898/2026.03.27.714759

Figure Lengend Snippet: A) Confocal imaging of immunofluorescence antibody staining of TH (magenta) and RFP (green) limited viral rescue of TH depleted neurons in Slc6a3 Cre ;Th Flox cKO mice. Overlap shows the co-expression (white) of these markers in cKO mice injected with AAV-DIO-TH-P2A-RFP into the SN. B) Confocal imaging of immunofluorescence antibody staining of TH (magenta) and GFP (green) negative control neurons in Slc6a3 Cre ;Th Flox cKO mice. Overlap shows the co-expression (white) of these markers in cKO mice injected with AAV-DIO-EGFP into the SN. C) Comparison of Midbrain SN TH+ cell counts per brain section in AAV-GFP (black) control mice and AAV-RFP-TH (orange) rescue mice. D) Normalized body weight of TH rescue and negative control Slc6a3 Cre ;Th Flox cKO mice over the course of the study; weight was normalized by dividing the weekly weight by the Day 0 weight, which was taken prior to beginning CR. E) Normalized food intake of TH rescue and negative control Slc6a3 Cre ;Th Flox cKO mice while on AL; data is normalized by dividing total AL raw food intake by initial body weight. F-H) Normalized high activity on days –7 (F), 21 (G), and 28 (H); data is normalized by dividing the seconds of high activity in the premeal window by the total daily high activity. I) Total daily high activity in seconds throughout the course of the study. J) High activity in seconds during the 3-hour premeal window throughout the course of the study. K) Normalized high activity during the 3-hour premeal window; data is normalized by dividing the seconds of high activity in the premeal window by the total daily high activity. Data are mean +/- SEM. Statistical significance of behavior was determined using Sidak’s Multiple Comparisons Test. Statistical significance of cell counts, feeding and weight were determined using unpaired Welch’s T test. ∗p < 0.05, ∗∗ p < 0.01, *** p < 0.001

Article Snippet: Slc6a3 Cre -driver mice were obtained from the Jackson Laboratory (Bar Harbor, ME; stock #020080); this line contains a gene insertion of Cre recombinase upstream of Slc6a3 .

Techniques: Imaging, Immunofluorescence, Staining, Expressing, Injection, Negative Control, Comparison, Control, Activity Assay

Journal: Cell reports

Article Title: Emotional words evoke region- and valence-specific patterns of concurrent neuromodulator release in human thalamus and cortex

doi: 10.1016/j.celrep.2024.115162

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: B6.SJL- Slc6a3 tm1.1(cre)Bkmn /J , Jackson Labs , RRID:IMSR_JAX:006660.

Techniques: Virus, Recombinant, Software, Isolation